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Thus, with pathologic HNE concentrations (10μM and higher) for rat GCLC, ERK activation was required for the HNE-mediated induction but for human GCLC, JNK activation was essential and Nrf2 was not required (Iles and Liu, 2005)

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Be sure to introduce restriction sites at the ends of the target gene fragment that are in-frame and complementary to the vector chosen